Figure. 4. Knockdown of KLF5 by siRNA attenuates LPA-mediated cell proliferation.
A, SW480 cells were transfected with siRNA specific for KLF5 (siKLF5) or control siRNA (siCont). The levels of KLF5 proteins were determined by Western blot. B and C, the effects of KLF5 knockdown on the rate of proliferation of SW480 (B) and HCT116 (C) cells were determined. Cells transfected with control or KLF5 siRNA were treated with 10 μM LPA for 3 days. Numbers of cells were counted by using a hemacytometer. Three independent experiments were performed with triplicate samples. Results presented are mean ± S.E. D, serum-starved SW480 cells transfected with control of KLF5 siRNA were treated with 10 μM LPA for 72 h. Cells were trypsinized, collected, and subjected to flow cytometric analysis. Experiments were repeated twice with quadruple samples. *, p < 0.0001; **, p < 0.05 compared with control.