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ChIP analysis of SRF binding to the endogenous CArG regions. PCR was carried out to detect the endogenous CArG regions in immunoprecipitated chromatin fragments. Lanes 1, 4, 7, and 10 show PCR amplification of control precipitation samples with no Ab. Lanes 2, 5, 8, and 11 show amplification of 1:100 dilution samples of total input DNA for immunoprecipitation. Lanes 3, 6, 9, and 12 show amplification of target sequences in immunoprecipitated chromatin fragments with anti-SRF Ab.
