Figure 1. Identification of rCGG repeat-binding proteins (RBPs).
A. Gel shift assay with mouse brain lysates. Lane 1, rCGG probe only; Lane 2, rCGG probe with mouse brain lysates; Lanes 3-7, rCGG probe and mouse brain lysates with the indicated increasing amounts of unlabeled rCGG repeats (molar ratio). B. Gel shift competition assay with fly brain lysates. Lane 1, rCGG probe only; Lane 2, rCGG probe with fly brain lysates; Lanes 3-5, rCGG probe and fly brain lysates in the presence of 100-fold unlabeled triplet repeat RNA, as indicated (molar ratio). C. Identification of RBPs. Coomassie Blue staining gel with RBPs is shown, and distinct bands were cut for protein identification. The identities of those proteins are indicated on the right.
