Skip to main content
. Author manuscript; available in PMC: 2007 Oct 15.
Published in final edited form as: Dev Biol. 2007 Jan 8;304(2):479–498. doi: 10.1016/j.ydbio.2007.01.001

Figure 8. Synchronized Notch signaling inactivation reveals an inherent proneural bHLH cascade during progenitor cell differentiation.

Figure 8

QPCR was used to analyze changes in E4.5 chick retinal explant pairs treated with DAPT compared to control over time as before. Cash1 (A), Ngn2 (B), NeuroM (C), NeuroD (D), and Cath5 (E) all exhibit statistically significant dynamic temporal changes over time due to Notch signaling inactivation. (F) Comparing the relative patterns of changes reveal that three distinct transient and sequential responses: (1) Cash1 and Ngn2 were transiently upregulated at 3h, with Cash1 peaking at 6h and Ngn2 peaking at 12h, and both downregulated to below normal levels by 48h; (2) NeuroM transiently increased later at 6h, peaked at 12h, and decreased to normal levels by 48h; (3) NeuroD and Cath5 increased even later sometime after 6h, with both peaking at 12h: Cath5 decreased back to normal levels while NeuroD remained elevated by 48h (Fig 7 D, E respectively).