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. Author manuscript; available in PMC: 2007 Oct 15.
Published in final edited form as: Dev Biol. 2007 Jan 8;304(2):479–498. doi: 10.1016/j.ydbio.2007.01.001

Figure 6. Notch signaling inactivation promotes stage appropriate neuronal differentiation.

Figure 6

(A–F) DAPT treatment of E12.5 mouse retina increased ganglion cell differentiation, visualized by Tuj1 and Islet1 (Isl1) immunolabeling of sections (A, B and D, E) and wholemounts (C) and (F) respectively: asterisk in (C) and (F) mark DAPT-treated explant. (G–J) DAPT treatment at E12.5 also increased cone photoreceptor differentiation visualized by Trß2 immunolabeling (G, H), but not later-born rod photoreceptors visualized by lack of Rhodopsin immunoreactivity in both control and DAPT-treated explants (I, J respectively). (K–R) DAPT treatment at P1 had no effect on earlier-born Tuj1+ neurons (K, L), but produced a clear increase in both Recoverin and Rhodopsin immunolabeling of rod photoreceptors in the apical region of the outer nuclear layer (M–P); also note the numerous newly generated rods in the more basal region of the outer nuclear layer (arrowheads, N, P). DAPT treatment also produced a clear decrease in the differentiation of Muller glia cells, which have just begun to differentiate as visualized by CRALBP and Cyclin D3 immunolabeling (Q, R).