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. Author manuscript; available in PMC: 2007 Oct 15.
Published in final edited form as: Dev Biol. 2007 Jan 8;304(2):479–498. doi: 10.1016/j.ydbio.2007.01.001

Figure 3. Loss of Notch signaling synchronizes neuronal differentiation.

Figure 3

(A, B) E4.5 chick retinal explants pairs cultured for 48h in DAPT or DMSO were wholemount immunolabeled with anti-Visinin antibody to reveal differentiating cone photoreceptors in the apical retina: images were acquired from flatmounted explants with LSCM. (C–E) QPCR was used to quantify changes in gene expression levels of Visinin (C) and RXR-□ (D), both of which are specifically expressed in differentiating cones in chick, and Nell2 (E), which is generally expressed during neuronal differentiation, including ganglion cells and photoreceptors (Nelson et al., 2002; Nelson and Reh, unpublished observations). (F) Relative comparison of changes in neuronal gene expression over time as described before. Note that the general neuronal differentiation gene is upregulated by 12h after Notch signaling inactivation, while cone specific genes are upregulated after 24h.