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. 2007 Jul 1;65(2):277–293. doi: 10.1111/j.1365-2958.2007.05813.x

Fig. 1. Primer extension analysis of rpoS transcripts.

Fig. 1

A. Schematic of the B. burgdorferi rpoS region indicating flgI (black arrow), flgJ (red arrow) and rpoS (blue arrow); the approximate locations of putative promoters (small bent black arrows) are indicated. The gene names are as annotated in the B. burgdorferi B31 genome sequence (TIGR and/or ERGO, Integrated Genomics). The DNA sequence in the region between flgJ and rpoS is shown. The stop codon (TAA) for flgJ is indicated in red, and the start codon (ATG) for rpoS is indicated in blue. The σ54-dependent promoter for rpoS is highlighted with a box around it, and the conserved −24/−12 GG/GC doublets are shown in bold. The transcriptional start site for this promoter is indicated in bold with an asterisk below it.

B. Primer extension reactions were performed with RNA extracted from B. burgdorferi strains B31-A (1) and B31-ARpoN (2) at 2 × 108 cells ml−1. The sequencing ladder was generated with the rpoS-4 primer. The −24/−12 doublets are indicated, and the +1 site is indicated by an arrowhead (<) denoting the 5′ end of the transcript.