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. Author manuscript; available in PMC: 2007 Sep 10.
Published in final edited form as: Virology. 2007 Feb 5;362(2):331–341. doi: 10.1016/j.virol.2006.12.014

Fig. 2.

Fig. 2

VLPs induce in vitro maturation of DCs. (A) Gating of CD11c+ DC populations. DCs expanded in vivo by injection of mice with the FL plasmid DNA were purified on CD11C+ magnetic beads. (B) Flow cytometry profile of DC activation markers of purified DC populations. DCs were obtained from spleens of mice that received the FL plasmid DNA, purified as in A, and DCs (1X106/ml) were incubated with VLPs (10μg/ml) for overnight. Stimulated DCs were characterized for DC maturation in vitro after staining the cells with antibodies against surface markers CD40, CD80, and CD86. C) Mean fluorescent intensity (MFI) for DC activation markers CD40, CD80 and CD86 induced by various VLPs was derived from the panel B and presented. These data are representative from three independent experiments.