Figure 8.
HSF1 redox activation protects cells from stress-induced apoptosis. Hsf1−/− stable cell lines harboring the empty vector (V), wild-type HSF1 (WT), or the indicated mutant derivatives were incubated under control conditions (37°C), exposed to heat shock (44°C for 1 h followed by 6 h at 37°C), or 500 μM H2O2 at 37°C for 6 h. Relative apoptosis of these cultures was determined as described in Materials and Methods and plotted on the y axis as the Relative Apoptosis index.
