Figure 3.
Anti-xNdc80- and anti-xNuf2-antibody-injected cells bypass the spindle checkpoint. Mitotic XTC cells, treated with taxol, were injected with either anti-xNdc80 or anti-xNuf2 antibodies. After an additional 2 h, the cells were fixed, immunolabeled with fluorescent anti-rabbit IgG secondary to locate injected cells, and stained with DAPI. (A) Examples of the two major phenotypes resulting from anti-xNuf2 antibody injection in taxol. There were either multinucleated polyploid cells or cells with a “cut” phenotype. The insets show the injected antibody detected by fluorescent anti-rabbit IgG secondary. (B) Quantification of injected cells. The numbers above the bars indicate the number of injected cells in each category.