TABLE 2.
GenoType MTBDRplus test results in comparison with GenoType MTBDR test results for detection of MDR, INH-resistant, and fully susceptible strains in smear-positive sputum specimens
| Specimena (n) | No. (%) of strains | Sequencing data, nucleotide/amino acid change(s) | MTBDR assayb
|
MTBDRplus assayb
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| RMP pattern (rpoB) | INH pattern (katG) | Resultc | RMP pattern (rpoB) | INH pattern (katG) | INH pattern (inhA) | Result | |||
| MDR (31) | 19 (61.3) | Not done | ΔWT 5, Mut 3 | ΔWT, Mut T1 | MDR | ΔWT 8, Mut 3 | ΔWT, Mut T1 | WT | MDR |
| 1 (3.2) | Not done | ΔWT 5, Mut 3 | ΔWT, Mut T1 | MDR | ΔWT 8, Mut 3 | ΔWT, Mut T1 | ΔWT 1, Mut C15T | MDR | |
| 1 (3.2) | rpoB S531L, katG WT, inhA WT, ahpC WT | ΔWT 5, Mut 3 | WT | RMPr | ΔWT 8, Mut 3 | WT | WT | RMPr | |
| 1 (3.2) | rpoB S531L, katG S315N | ΔWT 5, Mut 3 | ΔWT | MDR | ΔWT 8, Mut 3 | ΔWT | WT | MDR | |
| 1 (3.2) | rpoB S531L/WT mix, katG S315T/WT Mix | WT (5), Mut 3 | WT, Mut T1 | MDR | WT (8), Mut 3 | WT, Mut T1 | WT | MDR | |
| 1 (3.2) | rpoB WT, katG S315N | WT | ΔWT | INHr | WT | ΔWT | WT | INHr | |
| 3 (9.7) | rpoB H526D, katG S315T | ΔWT 4, Mut 2b | ΔWT, Mut T1 | MDR | ΔWT 7, Mut 2b | ΔWT, Mut T1 | WT | MDR | |
| 3 (9.7) | rpoB H526D, katG S315T | ΔWT 4, Mut 2B | ΔWT, Mut T1 | MDR | ΔWT 7, Mut 2B | ΔWT, Mut T1 | ΔWT 1, Mut C15T | MDR | |
| 1 (3.2) | rpoB H526R/WT mix, katG S315N/WT mix | WT (4) | (WT), Mut T1 | MDR | WT (7) | (WT), Mut T1 | WT | MDR | |
| INHr (10) | 2 (20) | Not done | WT | ΔWT, Mut T1 | INHr | WT | ΔWT, Mut T1 | WT | INHr |
| 1 (10) | Not done | WT | ΔWT, Mut T1 | INHr | WT | ΔWT, Mut T1 | ΔWT 1, Mut C15T | INHr | |
| 3 (30) | katG WT, inhA WT, ahpC WT | WT | WT | Susc. | WT | WT | WT | Susc. | |
| 1 (10) | inhA C15T | WT | WT | Susc. | WT | WT | ΔWT 1, Mut C15T | INHr | |
| 1 (10) | rpoB L533P, katG S315T | ΔWT 5 | ΔWT, Mut T1 | MDR | ΔWT 8 | ΔWT, Mut T1 | WT | MDR | |
| 1 (10) | rpoB L533P, katG S315N | ΔWT 5 | ΔWT | MDR | ΔWT 8 | ΔWT | WT | MDR | |
| 1 (10) | rpoB WTd | WT (4, 5) | ΔWT, Mut T1 | INHr | WT (7, 8) | ΔWT, Mut T1 | ΔWT 1, Mut C15T | INHr | |
| Susceptible (30) | 30 (100) | Not done | WT | WT | Susc. | WT | WT | WT | Susc. |
a
Resistance was determined by conventional DST.
b
WT, wild-type pattern with all respective bands visible; Δ, missing bands; weak or very weak bands are listed in parentheses. Mut, mutation.
c
r, resistant; Susc., susceptible.
d
This isolate contained a second rpoB sequence derived from a mycobacterial strain not further characterized.