Figure 1.
Immunocytochemistry of PC12 cells survival and differentiation. (A) PC12 cells died when they were differentiated with hNGF and subsequently deprived of serum and hNGF. When infected with a lentiviral vector encoding Bcl-xL, PC12 cells differentiated with hNGF survived for an additional 3 weeks (B) (stained blue for tyrosine hydroxylase). PC12 cells not differentiated with hNGF and deprived of serum were dead after 3 days (C). However, PC12 cells infected with a vector containing Bcl-xL expressed Bcl-xL (green) and tyrosine hydroxylase (blue) and survived for 7 days (D).