Fig. 2.
E-cadherin promoter methylation analysis. E-cadherin-expressing and -negative cell lines were examined for promoter methylation. Only selected examples are shown. Lymphocyte DNA was used as a source of unmethylated DNA, and in vitro methylated DNA was used as a positive control. Cells were untreated, or treated for 24 h with Li+ (20 mM), TSA (0.33 μM), or both. After DNA extraction and sodium bisulfite treatment, PCR was performed for 35 cycles with primers specific for methylated (M) or unmethylated (U) templates, and the products were analyzed on agarose gels.