FIG. 2.
Schematic diagram of regions 1 and 2, which are unique to pK29. The boundaries between the regions and the known sequence from pR478 are indicated by dashed lines. The ORFs are shown as arrows, with the arrowheads indicating the direction of transcription. The β-lactamase genes are shown in black. The ORFs related to IS are shown in gray. Unmarked white arrows are hypothetical genes. IR, inverted repeats; IR*, incomplete inverted repeats. The genes and their products are as follows: sul-1, dihydropteroate synthetase; qacEdelta1, quaternary ammonium transporter protein; aadA2, streptomycin adenyltransferase; putative aac, putative aminoglycoside 6′-N-acetyltransferase (80% identical to AAC6-II of Acinetobacter baumannii [GenBank accession no. ABL95928]); catB2, chloramphenicol acetyltransferase; and intl1, integrase. In region 2, the ISEcp1 element to the left of blaCTX-M-3 is truncated (tnpA*). The region homologous to plasmid pCTX-M-3 is indicated.