Figure 3.
Cell-spreading assay, under normal conditions. In the cell-spreading assay, phenotypes were assessed by phase-contrast microscopy in live cells during the spreading phase (see Materials and Methods for detail). (A) The schematic diagram of the mutated/deleted Ena/VASP proteins represents the cell lines used in the assay. These proteins were stably expressed as EGFP-tagged form in MVD7 fibroblasts using retroviral vectors. The bar graph depicts percentage of cells displaying Smooth-Edged, Filopodial, and Ruffling phenotypes for each cell line. (B) The column bar graph represents the average percentages of the Smooth-Edged, Filopodial, and Ruffling phenotypes for groups containing or lacking wild-type EVH2 given in A. The average percentages of the three phenotypes in the EVH2-containing group were used for χ2-test for each line. The asterisks in A indicate statistical significance (p < 0.05).