Figure 7. DCs were not affected by DT treatment in CD169-DTR mice.
Wild-type (+/+) or CD169-DTR (+/CD169DTR) mice were injected with (+DT) or without (–DT) 10 μg/kg of DT. Spleens were obtained 4 days after DT treatment. (A) Cryosections of the spleen were stained with CD11c (DCs) and B220 (B cells). Original magnification, ×200. (B) Single-cell suspensions of splenocytes were stained with CD11c alone (total DCs) or in combination with CD8α (lymphoid DCs) or CD11b (myeloid DCs) and analyzed by flow cytometry.
