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. 1986 Jun;88(2):307–313. doi: 10.1111/j.1476-5381.1986.tb10206.x

Morphine attenuates cholinergic nerve activity in human isolated colonic muscle.

D E Burleigh, S J Trout
PMCID: PMC1916830  PMID: 2873856

Abstract

The action of morphine on cholinergic nerves in human sigmoid taenia coli muscle strips (taenia) was investigated using a radiolabelling technique. Basal release of tritiated material from taenia was increased by electrical field stimulation (EFS). This increase was tetrodotoxin (3.14 microM)-sensitive and calcium-dependent. Analysis of basal and stimulated release of tritiated material indicated that evoked release (i.e. stimulated minus basal) is almost entirely due to an increase in [3H]-acetylcholine ([3H]-ACh) output. Evoked release of [3H]-ACh was dependent on the current strength and could be greatly reduced by exposing taenia to hemicholinium (34.8, 87.0 microM) before and during incubation with [3H]-choline (4 microCi ml-1, 15 Ci mmol-1). Spontaneous activity, muscle tone and the motor response of taenia to EFS were unaffected by morphine. Evoked, but not basal, release of tritiated material was inhibited by morphine (1.32-13.20 microM) in a concentration-dependent manner. The inhibition of release was frequency-dependent and naloxone (0.28 microM)-sensitive. The possible relationship between the effects of morphine on cholinergic nerves in taenia muscle and its actions in vivo are discussed.

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Selected References

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  1. BUCKNELL A., WHITNEY B. A PRELIMINARY INVESTIGATION OF THE PHARMACOLOGY OF THE HUMAN ISOLATED TAENIA COLI PREPARATION. Br J Pharmacol Chemother. 1964 Aug;23:164–175. doi: 10.1111/j.1476-5381.1964.tb01576.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bennett A. Pharmacology of colonic muscle. Gut. 1975 Apr;16(4):307–311. doi: 10.1136/gut.16.4.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burleigh D. E. Evidence for more than one type of 5-hydroxytryptamine receptor in the human colon. J Pharm Pharmacol. 1977 Sep;29(9):538–541. doi: 10.1111/j.2042-7158.1977.tb11391.x. [DOI] [PubMed] [Google Scholar]
  4. Burleigh D. E., Galligan J. J., Burks T. F. Subcutaneous morphine reduces intestinal propulsion in rats partly by a central action. Eur J Pharmacol. 1981 Nov 5;75(4):283–287. doi: 10.1016/0014-2999(81)90555-0. [DOI] [PubMed] [Google Scholar]
  5. Burleigh D. E., Levine D. F., Motson R. W. A method for studying the actions of drugs on motility of vascularly perfused segments of human colon. Arch Int Pharmacodyn Ther. 1984 Nov;272(1):118–128. [PubMed] [Google Scholar]
  6. Burleigh D. E. Non-cholinergic, non-adrenergic inhibitory neurons in human internal anal sphincter muscle. J Pharm Pharmacol. 1983 Apr;35(4):258–260. doi: 10.1111/j.2042-7158.1983.tb02927.x. [DOI] [PubMed] [Google Scholar]
  7. Chowdhury A. R., Lorber S. H. Effects of glucagon and secretin on food- or morphine-induced motor activity of the distal colon, rectum, and anal sphincter. Am J Dig Dis. 1977 Sep;22(9):775–780. doi: 10.1007/BF01694507. [DOI] [PubMed] [Google Scholar]
  8. Cowie A. L., Kosterlitz H. W., Waterfield A. A. Factors influencing the release of acetylcholine from the myenteric plexus of the ileum of the guinea-pig and rabbit. Br J Pharmacol. 1978 Dec;64(4):565–580. doi: 10.1111/j.1476-5381.1978.tb17319.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Crema A., Del Tacca M., Frigo G. M., Lecchini S. Presence of a non-adrenergic inhibitory system in the human colon. Gut. 1968 Dec;9(6):633–637. doi: 10.1136/gut.9.6.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Duthie H. L., Kirk D. Electrical activity of human colonic smooth muscle in vitro. J Physiol. 1978 Oct;283:319–330. doi: 10.1113/jphysiol.1978.sp012502. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ehrenpreis S., Sato T., Takayanagi I., Comaty J. E., Takagi K. Mechanism of morphine block of electrical activity in ganglia of Auerbach's plexus. Eur J Pharmacol. 1976 Dec;40(2):303–309. doi: 10.1016/0014-2999(76)90067-4. [DOI] [PubMed] [Google Scholar]
  12. Gillan M. G., Pollock D. Acute effects of morphine and opioid peptides on the motility and responses of rat colon to electrical stimulation. Br J Pharmacol. 1980 Mar;68(3):381–392. doi: 10.1111/j.1476-5381.1980.tb14551.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. James M. K., Cubeddu L. X. Frequency-dependent muscarinic receptor modulation of acetylcholine and dopamine release from rabbit striatum. J Pharmacol Exp Ther. 1984 Apr;229(1):98–104. [PubMed] [Google Scholar]
  14. Marchbanks R. M., Israël M. Aspects of acetylcholine metabolism in the electric organ of Torpedo marmorata. J Neurochem. 1971 Mar;18(3):439–448. doi: 10.1111/j.1471-4159.1971.tb11971.x. [DOI] [PubMed] [Google Scholar]
  15. North R. A. Electrophysiology of the enteric nervous system. Neuroscience. 1982 Feb;7(2):315–325. doi: 10.1016/0306-4522(82)90269-x. [DOI] [PubMed] [Google Scholar]
  16. North R. A., Tonini M. The mechanism of action of narcotic analgesics in the guinea-pig ileum. Br J Pharmacol. 1977 Dec;61(4):541–549. doi: 10.1111/j.1476-5381.1977.tb07546.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. PAINTER N. S., TRUELOVE S. C. THE INTRALUMINAL PRESSURE PATTERNS IN DIVERTICULOSIS OF THE COLON. I. RESTING PATTERNS OF PRESSURE. II. THE EFFECT OF MORPHINE. Gut. 1964 Jun;5:201–213. doi: 10.1136/gut.5.3.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. PATON W. D. The action of morphine and related substances on contraction and on acetylcholine output of coaxially stimulated guinea-pig ileum. Br J Pharmacol Chemother. 1957 Mar;12(1):119–127. doi: 10.1111/j.1476-5381.1957.tb01373.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Parolaro D., Sala M., Gori E. Effect of intracerebroventricular administration of morphine upon intestinal motility in rat and its antagonism with naloxone. Eur J Pharmacol. 1977 Dec 15;46(4):329–338. doi: 10.1016/0014-2999(77)90227-8. [DOI] [PubMed] [Google Scholar]
  20. Paton W. D., Vizi E. S., Zar M. A. The mechanism of acetylcholine release from parasympathetic nerves. J Physiol. 1971 Jul;215(3):819–848. doi: 10.1113/jphysiol.1971.sp009500. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pert C. B., Snyder S. H. High affinity transport of choline into the myenteric plexus of guinea-pig intestine. J Pharmacol Exp Ther. 1974 Oct;191(1):102–108. [PubMed] [Google Scholar]
  22. Rand M. J., Story D. F., Wong-Dusting H. Effects of impromidine, a specific H2-receptor agonist and 2(2-pyridyl)-ethylamine, an H1-receptor agonist, on stimulation-induced release of [3H]-noradrenaline in guinea-pig isolated atria. Br J Pharmacol. 1982 Jun;76(2):305–311. doi: 10.1111/j.1476-5381.1982.tb09221.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sato T., Takayanagi I., Takagi K. Pharmacological properties of electrical activities obtained from neurons in Auerbach's plexus. Jpn J Pharmacol. 1973 Oct;23(5):665–671. doi: 10.1254/jjp.23.665. [DOI] [PubMed] [Google Scholar]
  24. Small R. C., Yong V. W. The failure of morphine to depress selectively non-adrenergic neural inhibition of the guinea-pig taenia caeci. J Pharm Pharmacol. 1983 Jan;35(1):54–56. doi: 10.1111/j.2042-7158.1983.tb04267.x. [DOI] [PubMed] [Google Scholar]
  25. Stewart J. J., Weisbrodt N. W., Burks T. F. Central and peripheral actions of morphine on intestinal transit. J Pharmacol Exp Ther. 1978 Jun;205(3):547–555. [PubMed] [Google Scholar]
  26. Vizi E. S., Ono K., Adam-Vizi V., Duncalf D., Földes F. F. Presynaptic inhibitory effect of Met-enkephalin on [14C] acetylcholine release from the myenteric plexus and its interaction with muscarinic negative feedback inhibition. J Pharmacol Exp Ther. 1984 Aug;230(2):493–499. [PubMed] [Google Scholar]
  27. Wetzel G. T., Brown J. H. Presynaptic modulation of acetylcholine release from cardiac parasympathetic neurons. Am J Physiol. 1985 Jan;248(1 Pt 2):H33–H39. doi: 10.1152/ajpheart.1985.248.1.H33. [DOI] [PubMed] [Google Scholar]
  28. Wikberg J. Release of 3H-acetylcholine from isolated guinea pig ileum. A radiochemical method for studying the release on the cholinergic neurotransmitter in the intestine. Acta Physiol Scand. 1977 Nov;101(3):302–317. doi: 10.1111/j.1748-1716.1977.tb06012.x. [DOI] [PubMed] [Google Scholar]

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