Figure 3.
Myc-mediated apoptosis caused by glucose deprivation appears to be independent of wild-type p53 activity. (A) A mouse embryo fibroblast cell line, (10.1)Val5-Myc (+Myc) that overexpresses c-Myc and lacks the wild-type p53 function at 39°C, underwent more extensive apoptosis caused by glucose deprivation when compared with (10.1)Val5 cells (−Myc) that do not overexpress murine c-Myc. (B) Mouse fibroblast cells were grown in DMEM with 10% FBS and deprived of serum (0.1% FBS) for 24 or 48 h before flow cytometric analysis for apoptosis.