Table 2.
Hepatitis B surface antigen (HBsAg)-specific proliferative responses of peritoneal exudate cells (PEC) from the chimeras at 1 and 2 weeks post-engraftment
| HBsAg-immunized NOD/SCID | Non-immunized NOD/SCID | ||||
|---|---|---|---|---|---|
| PEC harvested on | In vitro stimulation | Ct/min mean ±s.d. | SI | Ct/min mean ±s.d. | SI |
| Week 1 | (n = 3) | (n = 3) | |||
| HBsAg (3 μg/ml) | 4725 ± 995 | 2·6 | 3006 ± 926 | 0·9 | |
| PHA (3 μg/ml) | 26 816 ± 2605 | 14·6 | 24 776 ± 434 | 7·7 | |
| Blank | 1834 ± 470 3 | 210 ± 438 | |||
| Week 2 | (n = 3) | (n = 3) | |||
| HBsAg (3 μg/ml) | 2945 ± 1217 | 8·8 | 653 ± 33 | 1·7 | |
| PHA (3 μg/ml) | 10 667 ± 3253 | 32 | 7936 ± 1253 | 21 | |
| Blank | 334 ± 48 | 374 ± 75 | |||
PBL (1 × 107 cells/mouse) from an HBsAg-immune donor were transferred intraperitoneally into the pretreated NOD/SCID mice (n = 12). Six mice were immunized subcutaneously with 2 μg HBsAg the day after cell transfer, the other six mice received PBS as control. One and 2 weeks after engraftment three mice from each group were killed. PEC were collected, stimulated in vitro with HBsAg or phytohaemagglutinin (PHA), or left without stimulating antigen. The detailed data are shown as mean counts of triplicate cultures and as stimulation index (SI).