Fig. 4.
Splenocytes from hspE7-immunized mice produce IFN-γ upon restimulation. C57Bl/6 mice (three per group) were immunized subcutaneously in the scruff of the neck with either PBS, 1·4 nmol (h)E7 or 1·4 nmol hspE7 and then boosted 14 days later. Fourteen days after the boost, pooled splenocyte cultures were prepared and restimulated with medium, 1·4 μm glutathione-S-transferase (GST; as irrelevant antigen) or 1.4 μm (h)E7, as described in Materials and Methods. IFN-γ (□) and IL-5 (▪) content of 72-h culture supernatants was determined by ELISA and expressed as pg/ml ± s.d. of triplicate ELISA values.