Figure 3. Dnmt3a interacts with Cbx4 specifically.
(A) Amino acid alignment of the COOH boxes and their flanking regions of the Cbx family members. Identical residues are indicated by black shading and highly conserved residues by grey shading. (B) Schematic representation of Cbx proteins with their Dnmt3a-interacting abilities (upper panel, shown on the right) based on the data from yeast two-hybrid assays (middle panel). Cbx proteins were fused to GAL4AD and co-transformed into yeast strain AH109 with GAL4BD–Dnmt3a. The interactions were accessed by growth of colonies on the SD minimal medium (SD-L-T-A-H). The GAL4AD-Cbx and GAL4BD-Dnmt3a fusion proteins also contain an HA or a myc epitope tag respectively, which were used to confirm the expression of Cbx proteins and Dnmt3a in transformed yeast by Western blotting (bottom panel).