Fig. 2.
Line graphs showing mean effects of soluble epoxide hydrolase inhibitors (sEHI) on LPS-induced thermal hyperalgesia and mechanical allodynia. The legend for Figs. 2A–C is shown in the lower right panel. (A) Intraplantar injection of LPS (solid squares; n=8) significantly decreased thermal hind paw withdrawal latencies (TWL) during the entire 4-h testing period. The sEHIs AUDA-be (open circles; n=6) and IK 950 (open triangles; n=8) blocked thermal hyperalgesia, although AUDA-be was more effective. Vehicle-treated controls (open squares; n=8) showed no significant change in TWLs. (B) The effects of the sEHIs AUDA-be (n=4) and IK 950 (n=4) on LPS-induced thermal hyperalgesia were doses dependent when three doses were tested at the 4-h timepoint. (C) Intraplantar LPS injection caused a significant decrease in mechanical withdrawal threshold (n=8), which was also blocked by the sEHIs AUDA-be (n=8) and IK 950 (n=8). * AUDA-be group, significantly different from LPS-only group. † IK 950 group, significantly different from LPS-only group. # dose–response, significantly different from low (50 mg/kg) dose. Values are shown as mean±SEM.