FIG. 3.
Frequencies of CD4 and CD8 T cells producing IFN-γ, by intracellular staining, in response to medium alone or to the pool of HCV core peptides before and after the addition of the blocking Abs anti-TGF-β and anti-IL-10 or the appropriate isotype controls in one representative subject, 002P (HCV monoinfected). After 6 h of appropriate stimulation (in the presence of Golgi Plug added 1 h after the start of stimulation), PBMC were stained with anti-CD3-PE-Cy5, anti-CD4-ECD, and anti-CD8-PE-Cy7 and then fixed/permeabilized for intracellular staining with anti-IFN-γ-PE. The dot plots are gated on CD3+ cells. The corresponding isotype control staining was <0.04 (not shown).