FIG. 5.
Level of MIR2 expression correlates with degree of MHC-I and ICAM-1 downregulation and KSHV MOI. HeLa cells were transfected with vector alone or a full-length MIR2 construct containing a FLAG recognition site. MHC-I and ICAM-1 surface levels were measured at 24 h posttransfection. (A) MHC-1 and ICAM-1 surface expression are presented as histogram analyses for vector alone or the MIR2 total transfected population (ungated). (B) Expression of MHC-I and ICAM-1 for the three MIR2 expression gates, low, medium, and high, as based on detection by an anti-FLAG antibody. (C) Levels of MIR2 and alpha-tubulin proteins were compared in samples following infection with MOIs of 0, 10, 20, and 30 at 2 days after infection. This was compared to MIR2-transfected, uninfected cells as shown in the lane labeled MIR2 (24 h posttransfection).