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Infectivity of JFH-1 virus in parental Huh7 cell. Parental Huh7 cells were seeded at 1 × 104 cells/well and infected with JFH-1 virus at 3 to 800 ffu/well for 48 h in 96-well plates. Infected cells were visualized with immunofluorescence using anticore antibody (2H9), and the foci were counted. All experiments were performed in triplicate, and the data are shown as means ± standard deviations.
