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. 1999 Jan 4;189(1):103–110. doi: 10.1084/jem.189.1.103

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Two subsets of CD1d-autoreactive T cells. Residual CD4⁺ cells in the spleen of MHC II^−/− mice were enriched by panning against CD8 and Ig, sorted into NK1.1-positive and -negative subsets, activated for 5 d with anti-CD3 and IL-2 in vitro, and fused with BW5147αβ⁻ to obtain T hybridomas. Individual hybridomas were screened for CD1d-autoreactivity and for Vα14-Jα281 rearrangement.