Table 3.
Comparison of HA titer of influenza and baculoviruses with hemadsorption to insect and mammalian cells expressing HA.
| Hemagglutination titer | Hemadsorption (A540)1 | |||||||
| Influenza virus | Recombinant baculovirus2 | MDCK cells | Sf9-cells | |||||
| Human | Chicken | Human | Chicken | Human | Chicken | Human | Chicken | |
| OK/323/03 | 64 | 0 | 512 | 0 | 0.73 ± 0.07 | 0.03 ± 0.005 | 0.71 ± 0.02 | 0.26 ± 0.06 |
| OK/369/05 wt | 64 | 0 | 128 | 0 | 0.76 ± 0.02 | 0.20 ± 0.005 | 0.80 ± 0.09 | 0.26 ± 0.03 |
| OK/369/05 S138A | N/A3 | N/A | 64 | 0 | N/A | N/A | 0.82 ± 0.07 | 0.31 ± 0.08 |
| OK/372/05 | 64 | 32 | 64 | 0 | 0.74 ± 0.004 | 0.40 ± 0.01 | 1.05 ± 0.09 | 0.35 ± 0.06 |
1 Mean of 4 experiments ± standard deviation
2 Baculovirus was concentrated 10-fold before HA titration
3 N/A = not applicable