Figure 4.
Localization of mutant Lt GFP-JBP1 in JBP1 null trypanosomes. JBP1 null trypanosomes (JBP1 dKO) were transfected with the wild-type (WT) or the mutant (H189A, D191A, H239A, R255A) Lt GFP-JBP1 fusions, DAPI-stained and visualized live on agarose slides by microscopy. DAPI staining shows the location of the nucleus (N) and of the kinetoplast (mitochondrial DNA; K).
