Figure 2.
PCR analysis of preimplantation embryos from +/− × +/− crosses. (A) Nested PCR strategy for genotyping embryos up to the blastocyst stage. Cenpc primer pairs include AK–AL for first-round synthesis and AM–AN for second-round synthesis, giving a final product of 322 bp for the untargeted allele. β-geo primer pairs include GF1–GR1 for first-round synthesis and GF2–GR2 for second-round synthesis, giving a final product of 248 bp for the targeted allele. Abbreviations for restriction enzyme sites are (Xh) XhoI and (S) SalI. The black box indicates exon 8 of the mouse Cenpc gene (see Fig. 1A). (B) Nested PCR genotyping of day-3.5 embryos.