Figure 1.
Immunohistochemical demonstration of ERβ in human breast cancer by immunohistochemistry (A) and mRNA in situ hybridization (B). ERβ is expressed also in intraductal carcinoma (C), and in normal ductal epithelium (D). The expression of ERβ in normal ducts was confirmed by mRNA in situ hybridization (E). F and H demonstrate the specificity control of the immunostaining. Adjacent tumor sections were immunostained with or without pre-absorption of the ERβ antibody by the immunogen peptide. The nuclear immunoreaction is completely abolished after pre-absorption.