Table 1.
Summary of E-Cadherin Immunohistochemistry, LOH, and Somatic Mutation Analysis of the Carcinomas of the Family Members with an E-Cadherin Germline Mutation in Exon 3
| Carcinomas | Immuno- histochemistry with antibody | LOH* | Somatic mutation† | |
|---|---|---|---|---|
| HECD-1 | AEC | |||
| Proband | ||||
| Gastric carcinoma | ++‡ | ++‡ | − | − |
| Mother of the proband | ||||
| Gastric carcinoma | +§ | − | − | n.d. |
| Breast carcinoma | − | +¶ | n.d. | n.d. |
*For LOH, sequencing analysis of exon 3 from DNA isolated from laser microdissected tumor cells was performed.
†Somatic mutation analysis was performed by sequencing all exons of the E-cadherin gene from DNA of manually microdissected tumor cells.
‡++: positive immunostaining of the cell membrane and diffuse and granular cytoplasmatic staining of the tumor cells.
§+: reduced granular cytoplasmic staining pattern
¶+: reduced diffuse cytoplasmatic and nuclear staining
n.d., not done