FIG. 3.
In vivo infection with a GFP-tagged MMTV pseudovirus. GFP-tagged MMTV Env-pseudotyped recombinant murine leukemia virus (MMTV/GFP) was injected into the right hind footpads of mice; the left hind footpads served as uninfected controls. Mice were killed 24 h later, and single-cell suspensions were prepared from the draining and nondraining popliteal lymph nodes. Cells were stained with anti-CD3 (A), anti-CD19 (B), or anti-CD11c (C), and GFP association with each cell type was analyzed by FACS. The same experiment was performed after AZT treatment (D and E). These data are representative of three experiments with similar results.