Figure 3. Analysis of libraries and results from screens.
A.) Statistics for libraries assayed. B.-E.) Measures of the activities of the synthetic riboswitches identified in the high throughput screens. Each measurement is done in triplicate. For all panels: Right axis: β-galactosidase activity in the absence (open circle) or presence of theophylline (1-mM, closed circle). Activities are expressed in Miller units, and the standard errors of the mean are less than the diameters of the circles. Left axis: The activation ratio of the synthetic riboswitch (green bar), which is determined by taking the ratio of the activities in the presence and absence of theophylline.