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. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: J Cereb Blood Flow Metab. 2006 Sep 13;27(5):975–982. doi: 10.1038/sj.jcbfm.9600399

Figure 5.

Figure 5

Double immunofluorescent staining of phosphorylation of Akt (pAkt) (red) and of GSK3β (pGSK3β) (green), and NeuN (green) and pGSK3β (red) in the cerebral cortex 6 h after SAH. Nuclei were counterstained with DAPI (blue). pAkt-positive cells (A) and pGSK3β-positive cells. (B) were observed in the cerebral cortex. (C) An overlapped image shows that pAkt-positive cells colocalized with pGSK3β-positive cells. (D) NeuN-positive cells reveal neurons in the cerebral cortex. (E) pGSK3β immunohistochemistry shows pGSK3β-positive cells in the same view. (F) An overlapped image shows that pGSK3β-positive cells colocalized with neurons. Scale bars represent 50 μm.