Figure 3.
Initiation of transcription in slp1-repressed embryos. ChIP assays on chromatin from wild-type and R+F embryos using anti-TBP antiserum (A), the monoclonal antibody 8WG16 (B), which recognizes the heptad repeats of the CTD, irrespective of their phosphorylation status (Patturajan et al. 1999; Jones et al. 2004; Morris et al. 2005), and the monoclonal antibody H14 (C), which specifically recognizes phosphorylation of serine residue 5 within the heptad repeats that comprise the CTD (Patturajan et al. 1999; Jones et al. 2004; Morris et al. 2005). Background controls for each antibody were determined using nonimmune serum from the appropriate species as indicated. The labeling scheme is the same as used in Figure. 2.