Figure 2.

Ryr mRNA is detected in developing visceral and hypodermal muscles. In situ hybridization was performed on wild-type embryos with DIG-labeled probes made from a partial Ryr cDNA. (A) A stage-11 embryo is shown in a lateral view, with dorsal up and anterior to the left. Expression of Ryr mRNA is strongest in the mesoderm. (B) A stage-14 embryo is viewed from the dorsal perspective, anterior to the left. Ryr expression appears widespread with high levels in hypodermal and visceral muscles.