Figure 4.
Alignment of automated DNA sequencing traces corresponding to bases 34 to 153 (sense strand) of the p27 gene. The location of unreactive (methylated) cytosines in bisulfite-modified DNA from NP and pituitary tumor cell lines are shown by the arrows. NP and AtT20 cells show complete bisulfite C to T conversion in this region. Eight methylated (unreactive) cytosines in GH3 control cells and seven methylated cytosines in GHRH-CL1 control cells were detected in this region. The number of methylated cytosines decreased to three and one in AZAdC-treated GH3 and GHRH-CL1 cells, respectively. Bisulfite conversion of the Hsp 92II restriction site is indicated. A: The sequence of p27 gene in GH3 cells without bisulfite reaction; B: NP; C: GH3 cells without AZAdC treatment; D: AZAdC-treated GH3 cells; E: GHRH-CL1 cells without AZAdC treatment; F: AZAdC-treated GHRH-CL1 cells; G: AtT20 cells.