Figure 2.
Oxidative damage occurs within the ipsilateral dLGN after occipital cortex ablation, as determined by immunodetection of OH8dG. A and B: The detection of OH8dG immunoreactivity within cells (arrows) is widespread within the focus of axotomy-induced neurodegeneration in the ipsilateral dLGN. OH8dG immunoreactivity is primarily confined to the nucleus and is detected as small, granular immunoreactivity (arrowhead) or as large, condensed aggregates of immunoreactivity (arrows). B: At higher magnification, the nuclear OH8dG immunoreactivity is localized diffusely within the periphery of the nucleus as well as at the surfaces of round aggregates, corresponding to the round, condensed clumps of chromatin that are detected by cresyl violet staining (see Figure 1A ▶ ). Scale bar = 5.5 μm. C: The control dLGN (ie, contralateral of the occipital cortex ablation) has no OH8dG immunoreactivity. D: Preadsorption of the OH8dG monoclonal antibody with purified OH8dG abolishes the nuclear OH8dG immunolabeling within the ipsilateral dLGN. Scale bar = 25 μm (applies also to A and C).