Figure 4.
Med12, but not Cdk8 or CycC, activity is required for bab2 expression in leg discs. (A–D) Leg discs from L3 harboring clones of Cdk8− (A), CycC− (B), Med12− (C) or Cdk8− Med12− (D) cells. Mitotic clones were generated using Dll>Flp. The left panels show clones of mutant cells (black, GFP−; representative examples are circled) in a background of wild-type (GFP+, green) cells. The right panels show bab2-expressing cells (red), as revealed by anti-Bab2 antibody staining. Nuclear Bab2 is autonomously reduced in Med12− single or Cdk8− Med12− double mutant cells, but is not modified in Cdk8− or CycC− cells.