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. 2007 Mar 16;8(4):414–419. doi: 10.1038/sj.embor.7400935

Figure 2.

Figure 2

Arachidonic acid triggers a rapid change in syntaxin 1 intrinsic fluorescence. (A) Fluorescence spectra of syntaxin 1, SNAP25 and synaptobrevin±arachidonic acid (AA) with excitation at 280 nm. The peaks reflect either tyrosine (syntaxin 1; Stx1) or tryptophan fluorescence (SNAP25 and synaptobrevin; Syb). (B) Decrease in syntaxin 1 fluorescence at 308 nm occurs within 30 s of addition of arachidonic acid. (C) Neither saturated fatty acids nor lysophosphatidylcholine (LysoPC) affect syntaxin 1 structure in the same way as arachidonic acid. SNAP25, synaptosomal-associated protein 25 kDa.