Figure 2.
Cells within terminal bronchioles support regeneration of airways of appropriate cellular diversity. CCSP-immunopositive cells in terminal bronchioles were detected by immunohistochemistry 45 days after intraperitoneal injection of vehicle (A) or 275 mg/kg of naphthalene (B). Nuclei were counterstained with hematoxylin; arrows indicate the BADJ (A and B). Morphometric analyses of cell density (total cells/100 μm basement membrane, C), and representation of Clara cells (percent Clara cells/total epithelial cells, D) were performed as described in Materials and Methods. Eight to 16 terminal bronchioles were analyzed per lung (n = lungs from three mice). Significant changes in overall cell density (C, ✻, P < 0.005) but not percentage of Clara cells (D) indicates an appropriate differentiation potential of epithelial cells within terminal bronchioles after naphthalene-induced injury. Original magnifications, ×400.