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. 2001 Feb;158(2):555–562. doi: 10.1016/S0002-9440(10)63997-1

Figure 2.

Figure 2.

Double immunofluorescence staining of filamentous tau lesions in CNS neurons of 24-month-old Tg mice. A–D: Double label immunofluorescence of a spinal cord spheroid (A, B) and NFT-like intraneuronal tau lesion in entorhinal cortex (C, D) of a 24-month-old Tg mouse using the T14 mAb (A, C) and the exon 2-specific antibody (B, D). Only the NFT-like lesions in entorhinal cortex show exon 2 immunoreactivity. E–H: Double immunofluo-rescence staining of NFT-like intraneuronal tau lesions using the T14 mAb (E, G), the anti-NFL antibody (F), and the anti-β-tubulin antibody (H) showing that the tau immunoreactivity does not colocalize with NFL or β-tubulin. (I, J) Double label immunofluorescence staining of a NFT-like tau lesion with the 17026 anti-tau antibody (I) and the anti-ubiquitin mAb (J) showing colocalization of tau and ubiquitin. All photomicrographs are at the same magnification. Scale bar, 10 μm.