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Expression of MMP-13 mRNA in a cell line derived from vaginal premalignant lesion. UT-DEC-1 cells established from a premalignant vaginal intraepithelial dysplasia were maintained in Keratinocyte Serum Free Medium (passage 21, p21) or serum-free DMEM (passage 89, p89) without or with TNF-α (20 ng/ml) or TGF-β1 (5 ng/ml), as shown, for 24 hours. Total RNA was extracted and 15-μg aliquots were analyzed for expression of MMP-13 and GAPDH mRNA by Northern blot hybridizations.
