FIGURE 4.
RhoA modulates Smad phosphorylation. A, Monc-1 cells were treated with (+) or without TGF-β and simultaneously treated with (+) or without (−) Y27632 (10 μm) for the times indicated. Cell lysates were prepared, and Western blotting was performed using antibodies for phospho-Smad (p-Smad), total Smad (Smad), α-SMA (indicating SMC differentiation). B, Monc-1 cells were infected with (+) DNRhoA adenovirus for 48 h or left uninfected (−). Cells were then treated with (+) or without (−) TGF-β (5 ng/ml) for various times as indicated. Western blotting was performed using antibodies as indicated. α-Tubulin serves as an internal control for equal loading for both A and B.