Table 1.
Oligonucleotides used for detection of ILT, LAIR, and KIR genes by PCR and determination of polymorphic sites by sequence analysis
| Sequence | Specificity | |
|---|---|---|
| 1 | 5′ GCCACAATCACTCATCAGAGTA 3′ | ILT1 specific fwd |
| 2 | 5′ GTATCGCTGTTACTATGGTAGCG 3′ | ILT2 specific fwd |
| 3 | 5′ ATGATCCCCACCTTCACGGCT 3′ | ILT3 specific fwd |
| 4 | 5′ CAGCTTCCATGCCTTCTGGG 3′ | ILT3 specific rev |
| 5 | 5′ TCAGTATTACAGCCGCGCTCGG 3′ | ILT4 specific fwd |
| 6 | 5′ GGTGCTATGGTTATGACTCGCGCG 3′ | ILT6 specific fwd |
| 7 | 5′ ATGACCGCCGCCCTCACAGCCT 3′ | ILT9 specific fwd |
| 8 | 5′ GCAGAGCAGGGCATCATGGTGT 3′ | ILT10 specific fwd |
| 9 | 5′ AGCCTCCGAGTGTCCACACTG 3′ | LIR6 specific fwd |
| 10 | 5′ TTGGCAGACAGTCCAGATAACATC 3′ | LIR6 specific rev |
| 11 | 5′ TGTCGTGGCCCGCGGAGGC 3′ | LIR8 specific fwd |
| 12 | 5′ TGACTGACACAGCAGGGTCACG 3′ | ILT redundant rev |
| 13 | 5′ CGTGACCCTGCTGTGTCAGTCA 3′ | ILT4 sequencing primer |
| 14 | 5′ CGAAGCCATGAGTTGCACACTG 3′ | Marker 204 fwd |
| 15 | 5′ CAACCACAGCATCTGTAGGCTCC 3′ | Marker 204 rev |
| 16 | 5′ GGGGAGCCATGTGACTTTCGTG 3′ | LAIR fwd |
| 17 | 5′ GTCACTCTGCTCAGACCATTTAG 3′ | LAIR rev |
| 18 | 5′ TGATTGGGACCTCAGTGGTCA 3′ | KIR exon 7 redundant fwd |
| 19 | 5′ CCCAACRCAYRCCATGMTGA 3′ | KIR exon 1 redundant rev |
The KIR oligonucleotides were used for linking adjoining genes on haplotype 1. KIR genes were detected by using primer sets as described (16).