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. 2006 Dec 11;75(3):1079–1088. doi: 10.1128/IAI.01143-06

TABLE 1.

Mini-Mu transposition insertion mutants in S. aureus S30 showing significantly defective biofilm formation after 6 h growth on polystyrene plates

S30 Em-Mu mutant NCBI gene identification N315 locus taga Gene name(s) Gene product(s) or domain similarity(ies) Insertion site sequenceb Em-Mu orientationc PIAd WTAe
Ω8105 15926180 SA0461 mfd Transcription-repair coupling factor TTTCATACGTTTCTTTCAAATCATTGCGCA → ± +
Ω7462 15926315 SA0594 tagGH Intergenic region between tagH (teichoic acid translocation ATP-binding protein) and tagG (teichoic acid translocation permease) ATGATTGTATACTATAATGTATTTGTAATA ← + +
Ω332 15926363 SA0641 mgr/norR/rat Transcriptional regulator, MarR family GTTAGATTGTACTTCTTAAAAACTTTGTTA → ± +
Ω7937 15926423 SA0701 bfd1 HPf, GGDEF domain CTTAGGTAATGTAAAAGAATTTGATAGTAC ← ± +
Ω9135 15926604 SA0874 bfd2 HP, similar to multidrug resistance protein, major facilitator family TTGCATGGATATTATATTATTTTGTCGATG ← ± +
Ω7971 15926643 SA0909 fmtA Autolysis and methicillin resistance-related protein TCCATGGTTTAAAACATCTAAGCCTATCCC ← − −
Ω7639 15926643 SA0909 fmtA Autolysis and methicillin resistance-related protein AACAGTAAAGACTTGTCCAAAGAATCCCCC → − −
Ω7105 15926643 SA0909 fmtA Autolysis and methicillin resistance-related protein ACAACTACATACTTATCATTATAGTAAACA → − −
Ω7586 15926675 SA0940 bfd3 HP, predicted hydrolase of metallo-β-lactamase/β-CASP superfamily GCCATTGAATATCTTTATTTTGATTTAACT → ± +
Ω5690 15926799 SA1059 fmt Methionyl-tRNA formyltransferase CCTAATACACTTAATTTATCATGCATCGTA → − +
Ω904 15926804 SA1064 bfd4 HP, similar to yjeQ (engC) GTPase ATGGTCGTATCTAAATTGCGCAATATTCCC → − +
Ω5300 15926833 SA1093 bfd5 HP, similar to DNA topoisomerase I topA GCTACTAAAGAAACCGTCGATTACTTTCCT → ± +
Ω6900 15926838 SA1098 codY GTP-sensing pleiotropic transcription repressor TTCTTCCAACATTTGAATAATTCTTTGACT → − +
Ω6441 15926858 SA1118 bfd6 HP, predicted hydrolase of metallo-β-lactamase/β-CASP superfamily GCAATGATAACTTCCATATTAGCAGAAGCC → − +
Ω1105 15926884 SA1142 glpD Aerobic glycerol-3-phosphate dehydrogenase GCCGCCTTTTAGACCTTCTTTTTTAACTAA → ± +
Ω819 15926945 SA1197 tyrA Prephenate dehydrogenase GTTTTATTACTCAAGGTGATATCTTTCCAC → − +
Ω1084 15927073 SA1323 srrA Staphylococcal respiratory DNA-binding response regulator SrrA ATAATAATCGGTGTTTGTTTATGTTCACGC → ++ +
Ω4536 15927656 SA1885 bfd7 HP, predicted ATP-dependent RNA helicase, DEAD box family GCAGGTAGCTGAACAATTAAGAGAATTTAG → ± +
Ω8686 15927656 SA1885 bfd7 HP, predicted ATP-dependent RNA helicase, DEAD box family ATAGGCATACCACCGAATACAGTAACAACT → ± +
Ω7546 15927681 SA1909 atpF ATP synthase β chain TTCAAGTTTCTGTGCATTTAACTTAGCTTG → ± −
Ω1348 15928079 SA2288 gtaB UTP-glucose-1-phosphate uridyltransferase TTGGTTATCATTATTCATACGTTCAATCGC → + +
Ω3412 15928146 SA2354 bfd8 HP, putative membrane acyltransferase GTGATGAAATGAATCACTATCATAAGCTCC → − +
Ω6936 15928157 SA2364 bfd9 HP, putative DNA binding TetR superfamily transcriptional regulator TTCAATTCGTTGCCTATTCTCTTTTGCATC → − +
Ω4681 15928252 SA2459 icaA Intercellular adhesion protein A ACCATATTATATGATTGAGAATTTCAAACA ← − +
Ω8779 15928252 SA2459 icaA Intercellular adhesion protein A AATATGGTGCATCTTGATCAACGATAGTAT → ND ND
Ω3408 15928252 SA2459 icaA Intercellular adhesion protein A CTCAAACATCAAAATATATAAAGGAAACCT → ND ND
Ω598 15928252 SA2459 icaA Intercellular adhesion protein A ATCGAGATGATTTGCTCAAACATCAAAATA → ND ND
Ω2514 15928252 SA2459 icaA Intercellular adhesion protein A TTTATANAAGTCATAGTAAATGATGATAGT → ND ND
Ω4079 15928253 SA2460 icaD Intercellular adhesion protein D CAAAAGACACAAGATATAGCGATAAGCTGC → − +
Ω7762 15928253 SA2460 icaD Intercellular adhesion protein D ATTGTAAGAGAAACAGCACTTATCGCTATA ← ND ND
Ω8184 15928254 SA2461 icaB Intercellular adhesion protein B CAGGCAATATGATCAAGATACTCAACACTA → − +
Ω8138 15928254 SA2461 icaB Intercellular adhesion protein B CTGGCATTAAATTATCACCGTGTAAGAAAA ← ND ND
Ω8501 15928254 SA2461 icaB Intercellular adhesion protein B GCTCAAAAGCATCATCACTAATTAATCTAA → ND ND
Ω1974 15928255 SA2462 icaC Intercellular adhesion protein C GCGTGCAAATACCCAAGATAACAATAAACA → − +
Ω5319 15928255 SA2462 icaC Intercellular adhesion protein C ACTGTAAAACAATCCCATTAATATGTAAGG → ND ND
Ω8945 15928255 SA2462 icaC Intercellular adhesion protein C CTCAAAATAAAGAATTGCATGATAACAACG → ND ND
Ω5960 15928255 SA2462 icaC Intercellular adhesion protein C CTCAAAATAAAGAATTGCATGATAACAACG → ND ND
Ω2041 15928255 SA2462 icaC Intercellular adhesion protein C ATAACTAAATAACGTTCTAAGAAATTTAAT → ND ND
a

Open reading frame numbering (ordered sequence tag) from the N315 genome.

b

The first 30 nucleotides after the Em-Mu disruption site in the gene sequence are indicated.

c

Transcription sense of Em-Mu cassette relative to the insertion site.

d

Immunoreactivity to polysaccharide intercellular adhesion antigen was scored with polyclonal anti-PIA antibodies on whole-cell extracts. +, approximate wild-type expression level (≥80%); ++, above S30 wild-type expression level; ±, reduced expression; −, equivalent Ω 4681 icaA mutant expression level.

e

Wall teichoic acid extraction, polyacrylamide gel resolution and staining: +, wild-type expression level; −, SA113 tagO mutant expression level.

f

HP, hypothetical protein.