FIG. 2.
Repression of MIS promoter by TNF-α through the negative regulation of SF-1 transactivation. HeLa cells were transiently transfected with a reporter alone or along with 10 ng of SF-1, GATA4, or SF-1 expression plasmid. After 24 h of transfection, they were treated with TNF-α alone (in nanograms/milliliter) or together with anti-TNF-α antibody (in micrograms/milliliter) for 24 h. (A) Cells were cotransfected with MIS-Luc reporter. (B) Cells were cotransfected with SFRE-Luc reporter. All values represent the mean ± the SEM of at least three separate transfection experiments.