Table 3.
Expression of intracellular interferon-α in plasmacytoid DCs due to stimulation with herpes simplex virus-1
| Diagnosis | Numbers | Dose of HSV-1 (PFU/ml) | IFN-α+ cells in plasmacytoid DCs (%) | Mean fluorescence intensity | Levels of IFN-α (pg/ml) |
|---|---|---|---|---|---|
| Patients with chronic hepatitis C | 10 | 1 × 105 | 3·4 ± 2·2* | 339 ± 67 | N.T. |
| 5 × 105 | 9·9 ± 5·0* | 420 ± 69* | N.T. | ||
| 1 × 106 | 14·7 ± 6·5* | 460 ± 67* | 72·3 ± 74·1* | ||
| Normal controls | 10 | 1 × 105 | 9·5 ± 2·8 | 432 ± 96 | N.T. |
| 5 × 105 | 26·7 ± 6·0 | 578 ± 109 | N.T. | ||
| 1 × 106 | 35·0 ± 7·6 | 610 ± 109 | 520·7 ± 297·1 |
DCs were stimulated with various doses of plaque forming units (PFU) of herpes simplex virus (HSV)-1 for 5 h and the levels of expression of intracellular IFN-α in plasmacytoid DC were estimated from flow cytometric profiles. The levels of IFN-α in the culture supernatants were estimated by enzyme-linked immunosorbent assay. The results were expressed as percentage of IFN-α+ cells in plasmacytoid DCs, its mean fluorescence intensity and the levels of IFN-α in the culture supernatants. Data are shown as mean+-standard deviation.
*
P < 0·01 compared to normal controls of the same dose of HSV-1. N.T., not tested.