TABLE 2.
Summary of the results of identification of variola virus DNA extracted from 48 isolates by using the VARJ7R3P TaqMan probe on the Smart Cycler instrumenta
| Species | No. of isolates | No. of samples positive/no. of samples tested |
|---|---|---|
| Camelpox virus | 3 | 0/11 |
| Cowpox virus | 1 | 1/8 |
| Monkeypox virus | 3 | 0/10 |
| Ectromelia virus | 1 | 0/8 |
| Gerbilpox virus | 1 | 0/8 |
| Herpes virus | 3 | 0/13 |
| Human DNA | 1 | 0/3 |
| Monkey DNA | 1 | 0/3 |
| Myxoma virus | 1 | 0/3 |
| Rabbitpox virus | 1 | 0/3 |
| Raccoonpox virus | 1 | 0/5b |
| Rickettsia spp. | 2 | 1/8c |
| Skunkpox virus | 1 | 0/3 |
| Vaccinia virus | 5 | 0/17 |
| Varicella-zoster virus | 2 | 0/8 |
| Variola virus | 48 | 198/206d |
| Water | NAe | 0/5 |
a
The DNA concentrations ranged from 1 fg/μl to 1 ng/μl for variola virus DNA and 100 fg/μl to 1 ng/μl for all other DNAs.
b
The highest concentration (1 ng/μl) was positive on the LightCycler instrument.
c
Rickettsiae spp. were negative on the LightCycler instrument.
d
The values on the LightCycler instrument were 205/206.
e
NA, not applicable.