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Dynamic range of the assay. Regression analysis was performed on the Ct against log copy numbers. A total of 83 samples contained purified variola virus genomic DNA ranging in concentrations from 1 fg/μl (25 copies) to 10 ng/μl (25 × 106 copies). The actual and predicted values are shown with the linearity coefficient (r2 = 0.95; significance F = 6.7E-56).
